How Is Bacterial Vaginosis (BV) Diagnosed?

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Dr Thanushree, has her MBBS from Kanachur Institute of Medical Sciences, Mangalore
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Updated on 26 February, 2025
- Article was updated as part of our commitment to diversity, equity, and inclusion.

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Bacterial Vaginosis (BV) is a common vaginal infection caused by an imbalance of bacteria in the vagina. Diagnosing BV typically involves a combination of medical history review, physical examination, and laboratory tests. Here’s how BV is diagnosed:
Medical History Review
- Symptoms Assessment: The healthcare provider will inquire about your symptoms, such as unusual vaginal discharge (often thin, white, gray, or greenish with a strong fishy odor), itching, burning sensation during urination, and any discomfort in the vaginal area.
- Medical and Sexual History: They may ask about your medical history, including any past vaginal infections or sexually transmitted infections (STIs), contraceptive methods used (like condoms or vaginal douching), recent antibiotic use, and sexual activity history.
Physical Examination
Pelvic Exam: A pelvic examination is usually performed to visually inspect the vaginal walls, cervix, and vulva for any signs of inflammation, unusual discharge, or redness. During this exam, the healthcare provider may also collect samples for further testing.
Laboratory Tests

- Vaginal pH Testing: BV is often associated with an elevated vaginal pH (greater than 4.5). A pH test involves taking a sample of vaginal fluid and testing its acidity level using pH strips or a pH meter.
- Whiff Test: This test involves mixing a small amount of vaginal discharge with a potassium hydroxide (KOH) solution. A characteristic fishy odor (positive whiff test) indicates the presence of BV.
- Microscopic Examination: A sample of vaginal discharge may be examined under a microscope to check for the presence of clue cells. Clue cells are vaginal epithelial cells covered with bacteria, a key indicator of BV.
- Culture Test: In some cases, a vaginal culture may be done to identify specific bacteria present in the vagina and determine their sensitivity to antibiotics. However, this is less common for routine BV diagnosis.
Additional Considerations
- STI Testing: Since BV symptoms can overlap with other vaginal infections and STIs, such as trichomoniasis or yeast infections, additional tests may be conducted to rule out other conditions.
- Pregnancy:
Pregnant women with BV may be screened and treated differently due to potential risks to the pregnancy.
Diagnosing BV involves a comprehensive approach that considers symptoms, medical history, physical examination findings, and specific laboratory tests. It’s essential to consult a healthcare professional for accurate diagnosis and appropriate treatment, as untreated BV can lead to complications like pelvic inflammatory disease (PID) or increased susceptibility to other STIs.
